Expresion de telomerasa, KI-67 y el Virus del Papiloma Humano oncogénico en neoplasias intraepiteliales cervicales

Abstract

Evaluate the relationship between Ki-67 and telomerase expression with CIN grade and the physical state of high risk human papillomavirus (HR-HPV) DNA. Methods. An observational comparative study was done of 80 fragments of paraffin embedded tissues, 20 corresponded to normal cervical tissue and 60 to cervical tissues with different CIN grades. Immunohistochemistry was used to detect the Ki-67 and telomerase proteins, and in situ hybridization with tyramide amplification was used to detect the presence of HR-HPV DNA